Free Software Crack Keygen Patch Serial Keys Full Version. Top 1. 0 Free Full version Antivirus Download. Antivirus has become the most essential need of every computer system. The virus and malware attacks are increasing every day, which is very harmful for the health of your PC. Hello, I am getting a notice of Win32:Alureon-FN [Rtk] malaware by my antivirus. Below is the hijackthis log as well the MBAM log I executed. Uninstall older version did not work either until removed the reg keys. by step instructions on how to download and Install AVG Free Antivirus. Step 6: Next. Also, there is a threat of hijacking the important data on your PC which may result serious consequences. If you want to protect your computer from these potential threats, then it is advised that get an antivirus that is capable of scanning every kind of latest as well as old virus. If you do not take the viruses on your PC seriously then it will slow down your machine and may also cause system crashes or whole data loss. There is a wide range of free antivirus software that can be downloaded and installed to protect the PC from any potential threat. For Every individual, Primary motive is PC protection, Protecting your computer from viruses and malware are the most important thing. Most of the viruses enter into your system from the internet. So, you should know how to stay protected online because only one peculiar virus could expose your financial information or stop your computer from working. ![]()
But the best and very first option is to protect your computer from these viruses by using antivirus. Luckily, some of the best antivirus software is completely free, some of them just free for trial and some for lifetime usage. Now you may be thinking that why are some of the very best antivirus programs free? Many popular antivirus companies offer free versions of their programs for home users while offering versions with more advanced features are premium based and are available in upgrade option for professional and business users which are featured here. Kaspersky Labs continues to gain popularity in the security software industry as this Anti- Virus has all the right features and tools, packed in an interface that is advanced but straightforward. ![]() All neccessesty features like Real- Time Protection against viruses, spyware, Trojans, worms, rootkits, botnets and more. Robust Anti- Spam protection , Small Hourly Signature Updates ,Two- Way Firewall to block hacker attack. Overall, Kaspersky Anti- virus 2. As one of the best antivirus software. Free download – only for 3. Avast Antivirus t provides great protection against viruses and spyware. Avast Free version,is an A- list freeware antivirus software, provides the same secure and fast protection of well- known, pricier antivirus programs. Avast is remarkable for its effectiveness. The antivirus, antispyware, and heuristics engines form a security core that also includes multiple real- time shields . Avast! has best- in- class strong self- protection built in, to make sure, no matter what you face, avast! And moreover avast comes compatible with both 3. Norton 2. 01. 1 was released recently, making this the latest Norton antivirus . Symantec Norton Internet Security 2. Facebook links and scareware. The new Facebook Scan checks links on your Facebook Wall and News Feed to see whether they link to malware or to sites known to harbour malwar. E this must catch your attention for sure as many virus come in your systems through social networking sites . Whether you are using Windows XP, Windows Vista, Windows 7 or even a Macintosh, Norton is right for you. Microsoft Security Essentials provides extreem real- time protection for your system that protects your system against viruses, spyware, and other malicious software. Microsoft Security Essentials runs quietly and efficiently in the background so that you are free to use your Windows- based PC the way you want—without interruptions or long computer wait times. Anti- malware testing group AV- Comparatives. Microsoft Security Essentials a top rating for malware removal, but now they’ve given it their best ranking in their performance test as well. This is enough to say, this is a microsft product and thus it is a reliable tool aswell . Competent antivirus software with identity theft protection from Identity Guard. AVG is a good name in the antivirus market, and just like the most important security solution developers on the market, also released an Internet Security suite. The latest version of the suite brings a total of 1. The virus signature database od avg is dynamically improved, regularly updated with the newest signatures . ANTI- VIRUS FREE EDITION Includes Core protection and surfing , searching and social network security other features are paid. Comodo Antivirus Keep Your PC Free of All Malware, Spyware, Viruses and Trojans Breakthrough, patent- pending technology prevents all infections . Award- Winning Firewall Keeps Personal Information Private Blocks unauthorized access, unknown threats, even hacker attacks. The biggest plus of this antivirus is Trust. Connect Wi- Fi Data Encryption for defense against wireless information theft. Installation of Comodo Antivirus is relatively painless – there’s no need to register the product and your system will require a reboot to finish the installation process. Some features are paid if you need full strength security. Mc. Afee is one of the biggest leading names in security software, and their desktop antivirus program, there are some outstanding qualities that make Mc. Afee antivirus a compelling security solution. Scheduling scans and tweaking settings should be no trouble for most users. The main interface provides simple navigation. All elements of the software are easily accessible from the main console. Free Trial download last 3. Days. Trend Micro is a Another good antivirus and antispyware combo package with IM and inbound/outbound email scanning. And is compatible with Microsoft Windows 7, Microsoft Windows XP , Microsoft Windows Vista(3. Symbian and Windows Mobile devices. But Overall, Trend Micro’s Anti. Virus + Anti. Spyware is good, but not great. The level of protection isn’t as comprehensive as we’ve seen from competitors. Free Trial download last 3. Days. Avira Anti. Vir should remain on top of every free antivirus users’ list. Avira received the 5- star PCSL Award for July 2. The result outlines the continuity in Avira’s detection capabilities. Avira Anti. Vir Personal protects you from viruses, Trojans, worms, spyware, adware, and various other kinds of malware, making it the best tool and certainly one of the best free antivirus programs. Avira Anti. Vir Personal is compatible with Windows 7, Windows Vista, Windows XP and Windows 2. Avira also makes a version of Anti. Vir Personal for UNIX. Bit. Defender has the most comprehensive feature set and is completely flexible. The software is a little heavy on the resources used and therefore not too popular . One special tool that is worth mentioning is the secure gamer mode. Whether you’re playing a video game or in full- screen mode for a presentation or video, the gamer mode turns off any interruptions, but doesn’t sacrifice security. Conclusion: I am leaving totally up to you to select which one is the best antivirus for you. All the above antivirus software are totally free and you can download as per your requirement and your system configurations. Some of them are also available for Linux like bitdefender, AVG, Avast etc. Even after using the anti- virus, you somehow lost your data then don’t worry there are some data recovery tools present out there for Linux to help you out. If you are a chrome user then we recommend you to check it’s some of the best extensions for more secure and safe browsing.
0 Comments
![]() ![]() In 2017 most video game consoles look pretty similar: black boxes of varying size aimed at being as discrete as possible. The brands who manufacture and distribute. Thank you for your continued support of Deadspin. Be careful out there. Major Currency Pairs Forex Quotes - forex.tradingcharts.com forex.tradingcharts.com/quotes/major_pairs.html Forex quotes for Major Currency Pairs. · · Music video by Rick Astley performing Never Gonna Give You Up.The Uncanny Sound Illusion That Creates Suspense in Christopher Nolan's Movies. Ever notice how Christopher Nolan’s movies (Interstellar, Inception, The Prestige) feel like an anxiety attack? Well, maybe that’s overstating things a bit. But the director does have a knack for creating an unnerving degree of tension. Turns out he’s using a little bit of musical magic to do it. The magic is actually a science- based audio illusion called a Shepard tone. Named after psychologist Roger Shepard, a pioneer in our understanding of spatial relation, the effect sounds like an infinitely ascending or descending scale. ![]() The tones are constantly moving upwards or downwards, but they never seem to reach a pinnacle or nadir. This is accomplished by stacking scales on top of each other—typically one treble scale, one midrange, and one bass—with an octave in between, then playing them in a continuous loop. A Shepard tone is sometimes referred to as the barber pole of sound. You can even see the similarity, when you hear it and look at the spectrum view of a Shepard tone. Don’t listen to this too long, or you might lose your mind: Anyways, Christopher Nolan just loves this. With longtime collaborator Hans Zimmer, the acclaimed director has used a Shepard tone in almost every one of his films in the last decade. He even writes his scripts to match the effect. In a recent interview, Nolan explained how he used Shepard tones in his newest film, Dunkirk: The screenplay had been written according to musical principals. There’s an audio illusion, if you will, in music called a “Shepard tone” and with my composer David Julyan on “The Prestige” we explored that and based a lot of the score around that. And it’s an illusion where there’s a continuing ascension of tone. It’s a corkscrew effect. It’s always going up and up and up but it never goes outside of its range. And I wrote the script according to that principle. I interwove the three timelines in such a way that there’s a continual feeling of intensity. Increasing intensity. So I wanted to build the music on similar mathematical principals. Knowing this, you gain a deeper understanding of films like Interstellar, Inception, and The Prestige. It also explains why these films seem somehow inconclusive. A Shepard’s tone creates a conflict that can’t be resolved, just like Nolan’s plots.[Digg, Business Insider]. Torrentz will always love you. Farewell. © 2003-2016 Torrentz. ![]() Corel. DRAW x. 8 Keygen [Win 1. Is now available the Keygen for the new Corel. DRAW Graphics Suite X8 (v. Xforce. The keygen works with the trial version. If you find any problems, please leave us a comment with your Windows version. Corel. Draw x. 8 Keygen- xf(8/7/2. Corel. DRAW X8 Keygen. ![]() Version of Keygen: v. Release Date: 8/7/2. Compatible with: Windows 1. Windows 8, Win 8. Windows 7. Cracked by xforce- cracks. Xforce keygen corelx. A little of history. Hola si quería saber si alguien tenia alguna clave de windows 7 ultimate de 64 bits por que en el momento de la inestalacion use una qe me sirvio pero despues en. Rubber Firewall @RubberFirewal Blog. Autodesk 2017 2016 Direct Links. Adobe CC 2015 2014 Direct Links. In October 1. 99. Corel launched Corel. DRAW 7, the first of its line of software optimized to harness the power of Intel technology MMXTM. Version 7, designed for Windows 9. ![]() Windows NT 4. 0, began to integrate Corel PHOTO- PAINT Corel. DRAW, which resulted in a more homogeneous production environment. Corel. DRAW x. 8 screen. Subsequently, in October 1. Corel released version 8 for Windows 9. Windows NT 4. 0. Corel. DRAW 8 announced as the tool for professionals, was designed to offer users maximum productivity in the shortest possible time. The new version included, among other components, Corel PHOTO- PAINT 8 as well as advanced tools for Web and expanded file import options. Corel. DRAW 8 for Power Macintosh came to light in July 1. Corel made available to users a free version of Corel. DRAW 8 Limited Edition for Macintosh that could be downloaded from its website at www. January to May 2. This version provided a comprehensive solution enthusiasts Macintosh graphic design that did not need all the features available in the full product Corel. DRAW for professionals. New Vector Art Tools. In April 1. 99. 9, Corel announced the release of Corel. DRAW 9 Graphics Suite. This version offered numerous improvements for professionals, such as the ability to publish in PDF format support EPS and PSD file embedded ICC color profiles, several new palettes and palettes on screen. Corel PHOTO- PAINT 9 also part of the suite of graphic design. Capitalizing on its success with Linux, Corel led to this open source platform Corel. DRAW and Corel PHOTO- PAINT. The Corel. DRAW expected for Linux released in August 2. Corel PHOTO- PAINT for Linux is available as a separate product and free download linux. Corel. DRAW 1. 0 Graphics Suite released in November 2. The functions of Corel. DRAW 1. 0 Graphics Suite offer professional graphic designer, web designers and desktop publishing professionals more control over the creative process, increased productivity, text editing capabilities in several languages and internet connectivity, all integrated into an intuitive and easy to use interface. In addition to Corel. DRAW 1. 0 and PHOTO- PAINT 1. Corel. DRAW 1. 0 Graphics Suite includes the Corel RAVE ™ (Vector Effects animated) application, which allows users to create animations, change effects on direct editing components of animation in the timeline and changing create web graphics. Version 1. 1 was the last dual version to date, being the Corel. DRAW 1. 1 suite last edited for the Apple Macintosh platform in 2. Corel. Draw x. 8 new touchdraw. In August 2. 00. 3, the company Vector Capital acquired Corel Corporation, becoming from that time a private company focused on the development of its two main products, Corel. DRAW and Corel Word. Perfect. In February 2. Corel. DRAW, a version where engineers simplified and optimized the internal structure of the application, which resulted in a much more powerful, agile and stable program. In February 2. 00. Corel. DRAW suite for the development of version 1. Corel Corporation had more in mind than ever, the opinions of thousands of users of previous versions, making far the best version of Corel. DRAW to date and demonstrating the dedication of Corel to maintain its position at the forefront of artistic creation applications and remain in the service of design professionals worldwide sector. Corel Corporation Corel. DRAW X3 decided to call its thirteenth version using Roman numerals in their nomenclature (X = 1. Arabic numerals (3) thus following the current trend in naming software. Full Tutorial for Beginners to this X8 version. A curious fact worth mentioning that the graphic image of the X3 version used the figure of a chameleon to symbolize the evolution and the efforts made by Corel. DRAW to fit perfectly to the current needs of professional design. Internationally, the chameleon in question, according to official sources, named Carlos, by Hispanic origins of the President of the company Corel Corporation. In January 2. 00. X3 version, this version was called Corel. DRAW X4. The graphic image of Corel. DRAW X4 brought the return (stylized and updated) the mythical symbol multicolored balloon was originally Corel. DRAW. The recovery of this nostalgic element was very well received by experienced users or Power- Users of Corel. DRAW because it represents the experience gained over the years and the safe bet the company to continue to lead and innovate in the sector. Personally, I found very nice to see the “Corel balloon” to start the program, he reminded me of my professional beginnings and gives me confidence check how well the program has evolved, adapting to new times. How to create a stylish 3. D text effect. The first quarter of 2. Corel introduced Corel. DRAW X5, which retains the same style of nomenclature that combines Arabic numerals and Roman, a detail that sometimes is misleading as there are people who believe that it is version 5 program, when in fact it is version 1. For the development of X4 and X5 versions Corel Corporation founded the Corel Advisory Council (Advisory Council Corel) consists of a selection of Corel. DRAW experts from around the world. From the Corel Advisory Council we were able to witness all phases of Corel. DRAW project, reviewing and commenting functionality we would like to incorporate in future versions directly with the development team Corel. DRAW. It was a unique experience that demonstrates the interest of Corel Corporation to create a comprehensive program covering all the needs of a professional designer, including more specialized options. In February 2. 01. Corel Corel. DRAW version, and version 1. Corel. DRAW X6. In this version it included a new typesetting engine, versatile tools of styles and color harmonies, enhanced performance with support for multicore processors and 6. Corel. DRAW as Powerclip tool functions, pattern fills with transparency or Smudge the new interactive tool. In March 2. 01. 4 Corel introduced the 1. Corel. DRAW, Corel. DRAW X7, an optimized version for Windows 7 and Windows 8 6. With notable improvements in interface design with personalized workspaces and better performance in all applications. Highlighting new features Dynamic font preview, improved pattern fill tools or the ability to add QR codes among others. New key and enhanced features. System Requirements. Windows 1. 0, Windows 8, Windows 7 (6. OS is recommended)Intel Core Duo 1. GHz, Core i. 3 or AMD Athlon 6. X2 3. 80. 0+ at 2. GHz or higher. compatible graphics card with DXVA2 VLD mode with Vertex and Pixel Shader 2. MB VRAM minimum for hardware decoding acceleration. MB or more of VRAM VGA. Minimum screen resolution: 1. Sound card compatible with Windows. At least 6 GB of hard disk space for full installation. Support input and output devices. Cameras and camcorders that record to internal memory, memory card, DVD or hard drive. Phone, i. Pad, i. Pod Classic with video, i. Pod touch, Sony PSP, smartphones with Windows and Android. USB capture devices, PC cameras, webcams. Cards IEEE 1. 39. Fire. Wire for use with DV / D8 / HDV camcorders. DV cameras UVC (USB Video Class). IEEE 1. 39. 4 compatible with OHCI. Analog capture cards for analog camcorders (VFW and WDM support for Windows XP and Broadcast. Driver Architecture for Windows Vista and Windows 7). Analog capture device and digital TV (Broadcast Driver Architecture). Blu- ray Disc, DVD- R / RW, DVD + R / RW, DVD- RAM or CD- R / RW compatible with Windows. Xforce keygen corelx. Important note to crack: Remove or Rename the following files: Corel\Corel. DRAW Graphics Suite X8\Setup\RMPCUNLR. Corel\Corel. DRAW Graphics Suite X8\Font. Manager\RMPCUNLR. Corel\Corel. DRAW Graphics Suite X8\Draw\RMPCUNLR. DLLCorel\Corel. DRAW Graphics Suite X8\Connect. RMPCUNLR. DLLCorel\Corel. DRAW Graphics Suite X8\Capture\RMPCUNLR. DLLCorel\Corel. DRAW Graphics Suite X8\PHOTO- PAINT\HMPCUNLR. DLLDownload our Keygen for Corel. Draw x. 8Corel. Draw x. Keygen- xf(8/7/2. Zip pass: www. xforce- cracks. ![]() Why does Windows Update fail with code 8. F8. F? I installed a Windows 7 Enterprise on a Virtual Machine (Microsoft Virtual Server 2. The Windows is activated. The first thing I found was this, but I am definitely sure my timezone and time and date information is correct. The TLS protocol defined fatal error code is 70. Searching for that version I. Windows update 80072f8f server 2012 r2 The Windows SChannel error state is 105. · Windows Server 2012. Code 80072F8F! I Did All The Steps And Will Not. 85df-0ac36be099bb/windows-update-error-code-80072f8f-i-did-all-the. Error 80072efe or 80072f76, Windows Update If you receive Windows Update error 80072efe or 80072f76 while checking for updates. · Hi to everybody! I have a customer that is having a HP Proliant ML350 server with Windows 2012 R2. Server har is a DC, DNS and FileSharing as well. The. · Windows Update error 80072f8f SYMPTOM When running Windows Update, the error code. Time could not be synchronized with global Update Server. · Windows Server 2012; Windows 2012 R2 servers failing on Windows update with 80072F8F error. Thread, Windows. · Hi there, I have a fresh installed Windows Server 2012 R2. As soon as I try to install Windows Updates I get the following error: 80072F8F Then I trie. The Server was http:// Home Server 2012 Windows Update Error 80072f8f Contact. Windows Update Error 80072f8f FixStill I get a Code 8. F8. F Windows Update encountered an unknown error. When trying to "Check for updates". I know about this KB article from Microsoft. I also know about this question on Server. Fault. I tried everything there to no avail. Any other suggestions? Update Error 8. 00. F8. F. Hello Christoph, and welcome to Sysnative! Commonest cause of this error is an incorrectly set computer date & time. Please check the date and time, including the year. See here for further details: HOWTO: Fixing Windows Update Error Code 8. F8. F | Back in Hack. If that doesn't help, check the BIOS time, and if not that either, since this is a server, it could well be a problem with the network proxy server not allowing the authentication to happen. This is really rare though, and I've only seen it a couple of times. Since this only occurred on a clean install though, it's likely to be the system time. Windows Server technical content for IT Administrators - Windows Server. Windows Server is the platform for building an infrastructure of connected applications, networks, and web services, from the workgroup to the data center. Use the links below to view technical content for IT professionals for the different versions of Windows Server. Important. Do you have Windows running on your PC? ![]() Total Backup Recovery Key Features. Supports Windows 10/8/7/XP/Vista and Windows servers 2016/2012/2008/2003; Restore files and the whole system in minutes. Summary: It is possible to backup to NAS using Windows Server 2008 Backup. However, the in-built wizards in Windows don't allow for this. Instead, you need to script. Page 3, Introduction © 2016 Veritas Technologies LLC / 2016-12-15 Backup Exec™16 SCL Contents Getting Started Backup Exec Server Operating System Compatibility. As a reliable SQL Server backup software, EaseUS Todo Backup can do SQL backup and restore to protect MS SQL Server database in case of Server failure for SQL Server. Windows 1. 0, Windows 8 or 8. Windows 7? Do you have a problem? Go to Microsoft Support - just type your problem into the search bar. They have information about Windows, Office, Skype, you name it. The information below is only about Windows Server. Windows Server 2. Windows Server 2. Windows Server 2. R2 and Windows Server 2. Windows Server 2. R2 and Windows Server 2. Technical Library. Windows Server 2. R2 Developer Library on MSDNWindows Server 2. R2 and Windows Server 2. Windows Server 2. R2 and Windows Server 2. Technical Library. Windows Server 2. Developer Library on MSDNWindows Server 2. Windows Server 2. Technical Library - download a PDF version of the archived content. Windows Server 2. Developer Library on MSDNProduct evaluations. Download Windows Server 2. Evaluation. Download Windows Server 2. R2 Trial. Windows Server 2. Tip. Are you having a problem? Start with Microsoft Support - just type your problem into the search bar. You can get help with Windows, Office, Skype, you name it. Please enable cookies and refresh the page. Perhaps the single largest difference between Access and Excel is the method for record retention. Access records are free for modification at any time. ![]() Should You Integrate Microsoft Excel With A Microsoft Database. This is a BIG topic on BIG Data, but these are Excel ‘hints‘. The challenge here is saying a little, pointing you to a few in- depth resources, to possibly spark an interest or even to pinpoint a possible solution. So here in this post we will point to three extended posts we did on this topic, in addition to one link to our favorite Access resource and one to our favorite Excel resource, both run by Microsoft MVP’s. If you have used Microsoft Excel with BIG Data it is likely that you have encountered performance issues, and you have possibly experienced the worst problem, an Excel workbook that does not respond, it just sits there, dead in the water, looking at you. So imagine this, it is Friday afternoon on a three day holiday weekend. You have big plans for the weekend and at 2pm you need to grab your boat, a few friends, and you need to hit the highway to get ahead of the holiday traffic. Your CFO gave you permission to leave the office at 2pm, but in return you have promised your CFO that you would publish the monthly reports before you leave the office. In this scenario, when you click the “Publish Reports” button in your Microsoft Excel workbook you watch in horror to see MS Excel freeze. You shut down Excel and you try several more times, but nothing, you even restart the computer, you try it on your buddy’s PC, but no matter what you do your Microsoft Excel solution has become unable to process what data scientists call BIG Data, the massive tables you store in this workbook. You have so much data that it has become unresponsive. In this case you had to cancel the three- day weekend, and instead of drinking beers with friends as you relax in your boat you find yourself alone in the office, spending the weekend trying anything and everything you can to get your workbook to work. Everything you try points to the same solution, you need to integrate Microsoft Excel with Microsoft Access. Said another way, you need to use Excel as a front- end to an Access back- end. Is it worth knowing how to do this, and to actually be very proficient in doing so?
![]() ![]() This is something we have been doing for our clients since 1. This is one of the most common solutions we have been providing our clients for 2. So yes, knowing how to do this at the advanced level can make you a good amount of money. As such we recommend that you give it a shot. Our second most common solution is integrating Microsoft Excel with a SQL Server back- end, something our competitors are just now starting to do, but we are getting ahead of ourselves, that is our second post in the series. How common is this problem, having too much data in your Excel files that the performance suffers and the file takes 3. It is extremely common; BIG Data is nothing new, HUGE Data is now beginning to appear, we have several firms each week hire us to resolve their BIG Data performance issues. The client that wrote the testimonial below got lucky, we were able to provide a solution with just a handful of hours. For this client there was no need to build a new system, no need to spend all of that time and money. The current application works when the data- set is small, so all we needed to do was to move the data into an Access back- end, and use Excel based VBA to move the data between the two files, placing the results where they needed to be. While we are focusing on an Excel front- end with an Access back- end for this post, it is important to note that Microsoft has not just one database, but three or four (Four if you count Power BI). So when it comes to BIG Data performance issues, select the Microsoft database that best houses and manipulates the size of your data. For example if your database has 3 gigs of data, Microsoft Access will be ill suited for your solution as Microsoft Access has a 2 gig limit. SQL Server comes in several versions, each with its own size limits. Click here to compare the Microsoft Databases to each other and to Oracle. Access. SQL Server. Azure. Power BIEach of the Microsoft databases can and be integrated with all of the applications in the Microsoft Office Suite. This allows you to use VBA to create a fully automated solution. You can use any of the Microsoft databases as the back- end to an Excel front- end. For this post we are selecting Microsoft Access as the back- end database. In this scenario you move massive amounts of data that was originally stored in Microsoft Excel to Microsoft Access. The data moved out of Excel will populate one or more large tables in Microsoft Access (Less than the 2 gig limit). Microsoft Excel VBA (Visual Basic for Applications – Marcos to some) will then move data between the two applications as needed. In this solution Access will not only store the data but Access will also run one or more queries on the data, possibly populating or updating additional Access tables, and Access will return filtered data sets back to Excel. This minimizes the amount of data that is stored in Excel, thus dramatically reducing the size of the Excel file, while optimizing performance. That is it, it is that simple. Integrating Microsoft Excel with Microsoft Access will enhance performance and it will get you past those BIG Data blues. Below you will find two links that will take you to our extended posts on the series. If you are interesting in taking your Excel applications to the next level, harnessing BIG Data, then splitting your application into an Excel front- end, to a Microsoft database back- end, read each of these posts to see what might work best for you and your organization. Microsoft Excel MVP, Debra at Contextures. Send Excel Data to Access”. Her site is our favorite Excel resource on the web. You can also “split” an Access database into two files: According to Microsoft, Splitting a database can help improve its performance and reduce the chance of database file corruption. When you split a database, you reorganize it into two files — a back- end database that contains the data tables, and a front- end database that contains all the other database objects such as queries, forms, and reports. Each user interacts with the data by using a local copy of the front- end database. This site is about providing an Excel hint, not proving a complex solution. For that you can either take the time to learn how to do so, and build it yourself, or you can search the web for a firm to hire. At Excel hints we do both. If you would like to discuss your needs and if you would like to receive a quote on what the work would cost, please contact us to discuss your needs in detail. HowTos¶ HowTos. Installation. Operating Systems. CentOS; Debian; Fedora; FreeBSD; Gentoo; Mac OS X; Microsoft Windows; openSuse; Solaris; Ubuntu; Web- and. Logging Real Visitor IP Addresses: mod_cloudflare for Apache httpd. Because Cloudflare acts as a proxy, you will notice changes to the way that your website visitors. How install Apache 2.4, PHP 5.4 and MySQL 5.5.21 on Windows 7. Learn how to set up Apache as a reverse proxy server on CentOS to redirect HTTP traffic to an ASP.NET Core web application running on Kestrel. ![]() How to Secure Cent. OS 7 Server with Mod. Security. Mod. Security is an open source web application firewall which enables web application defenders to gain visibility into HTTP traffic and provides powerful rule sets to enhance high security and protection. It provides a full package with real- time web monitoring, logging and access control. The rule sets can be customized and managed according to the user preferences. The freedom to choose what to do is an essential advantage of Mod. Security and really adds to the context of an open source. With full access to the source code, we've the ability to customize and extend the tool to fit our needs. In this article, I'm explaining how to install and configure Mod. Security on a Cent. OS 7 server. Let's walk through the installation steps. First of all, I would like to verify the server settings, mainly the present Apache version and the modules installed.[root@server. VServer version: Apache/2. Cent. OS)Server built: Nov 1. Server's Module Magic Number: 2. Server loaded: APR 1. APR- UTIL 1. 5. 2. Install Mod Proxy Apache Centos 7 IptablesCompiled using: APR 1. APR- UTIL 1. 5. 2. Architecture: 6. 4- bit. Server MPM: preforkthreaded: noforked: yes (variable process count)Server compiled with..- D APR_HAS_SENDFILE- D APR_HAS_MMAP- D APR_HAVE_IPV6 (IPv. D APR_USE_SYSVSEM_SERIALIZE- D APR_USE_PTHREAD_SERIALIZE- D SINGLE_LISTEN_UNSERIALIZED_ACCEPT- D APR_HAS_OTHER_CHILD- D AP_HAVE_RELIABLE_PIPED_LOGS- D DYNAMIC_MODULE_LIMIT=2. D HTTPD_ROOT="/etc/httpd"- D SUEXEC_BIN="/usr/sbin/suexec"- D DEFAULT_PIDLOG="/run/httpd/httpd. D DEFAULT_SCOREBOARD="logs/apache_runtime_status"- D DEFAULT_ERRORLOG="logs/error_log"- D AP_TYPES_CONFIG_FILE="conf/mime. D SERVER_CONFIG_FILE="conf/httpd. You can use these command to identify the dynamically compiled modules enabled with Apache.[root@server. MLoaded Modules: core_module (static)so_module (static)http_module (static)access_compat_module (shared)actions_module (shared)alias_module (shared)allowmethods_module (shared)auth_basic_module (shared)auth_digest_module (shared)authn_anon_module (shared)authn_core_module (shared)authn_dbd_module (shared)authn_dbm_module (shared)authn_file_module (shared)authn_socache_module (shared)authz_core_module (shared)authz_dbd_module (shared)authz_dbm_module (shared)authz_groupfile_module (shared)authz_host_module (shared)authz_owner_module (shared)authz_user_module (shared)autoindex_module (shared)cache_module (shared)cache_disk_module (shared)data_module (shared)dbd_module (shared)deflate_module (shared)dir_module (shared)dumpio_module (shared)echo_module (shared)env_module (shared)expires_module (shared)ext_filter_module (shared)filter_module (shared)headers_module (shared)include_module (shared)info_module (shared)log_config_module (shared)logio_module (shared)mime_magic_module (shared)mime_module (shared)negotiation_module (shared)remoteip_module (shared)reqtimeout_module (shared)rewrite_module (shared)setenvif_module (shared)slotmem_plain_module (shared)slotmem_shm_module (shared)socache_dbm_module (shared)socache_memcache_module (shared)socache_shmcb_module (shared)status_module (shared)substitute_module (shared)suexec_module (shared)unique_id_module (shared)unixd_module (shared)userdir_module (shared)version_module (shared)vhost_alias_module (shared)dav_module (shared)dav_fs_module (shared)dav_lock_module (shared)lua_module (shared)mpm_prefork_module (shared)proxy_module (shared)lbmethod_bybusyness_module (shared)lbmethod_byrequests_module (shared)lbmethod_bytraffic_module (shared)lbmethod_heartbeat_module (shared)proxy_ajp_module (shared)proxy_balancer_module (shared)proxy_connect_module (shared)proxy_express_module (shared)proxy_fcgi_module (shared)proxy_fdpass_module (shared)proxy_ftp_module (shared)proxy_http_module (shared)proxy_scgi_module (shared)proxy_wstunnel_module (shared)systemd_module (shared)cgi_module (shared)php. Installation. Once verifying the Apache setup, you can install Mod. Security package from the Cent. OS base repo.[root@server. Loaded plugins: fastestmirror. Loading mirror speeds from cached hostfile* base: mirror. Resolving Dependencies- -> Running transaction check- -- > Package mod_security. Finished Dependency Resolution. Dependencies Resolved===============================================================================================================================================Package Arch Version Repository Size===============================================================================================================================================Installing: mod_security x. Transaction Summary===============================================================================================================================================Install 1 Package. This will install the mod_security on your server. Now we need to configure it on our server. Check and confirm the integration of the module to Apache. Check for the configuration file generated with the default set of rules. The configuration file will be located inside the Apache custom modules folder "/etc/httpd/conf. Jun 1. 0 2. 01. 4 mod_security. M | grep securitysecurity. Now restart the Apache and verify whether the Mod_security module is loaded on restart in the Apache logs.[root@server. Mon Apr 1. 8 0. 6: 2. AH0. 12. 32: su. EXEC mechanism enabled (wrapper: /usr/sbin/suexec)[Mon Apr 1. Mod. Security for Apache/2. Mon Apr 1. 8 0. 6: 2. Mod. Security: APR compiled version="1. Mon Apr 1. 8 0. 6: 2. Mod. Security: PCRE compiled version="8. Mon Apr 1. 8 0. 6: 2. Mod. Security: LUA compiled version="Lua 5. Mon Apr 1. 8 0. 6: 2. Mod. Security: LIBXML compiled version="2. Mon Apr 1. 8 0. 6: 2. AH0. 17. 57: generating secret for digest authentication ..[Mon Apr 1. AH0. 22. 82: No slotmem from mod_heartmonitor[Mon Apr 1. AH0. 01. 63: Apache/2. Cent. OS) PHP/5. 4. From the logs, you can identify the Mod. Security version loaded and other details. Identifying the Nature. We need to go through the Mod. Security configuration file to identify the include path for the custom rules which we can add for customization and also identify the log file path for further analysis. We can add the custom rules inside this path according to the configuration.# Mod. Security Core Rules Set configuration. Include. Optional modsecurity. Include. Optional modsecurity. Jun 1. 0 2. 01. 4 activated_rules. And we can inspect the log file at /var/log/httpd/modsec_audit. Customizing Mod. Security with the Core rule sets. We can get the custom rule sets from the official repo. These rule sets are automatically symlinked to the activated rules and make it effective on install by default. Loaded plugins: fastestmirror. Loading mirror speeds from cached hostfile* base: mirror. Resolving Dependencies- -> Running transaction check- -- > Package mod_security_crs. Finished Dependency Resolution. Dependencies Resolved===============================================================================================================================================Package Arch Version Repository Size===============================================================================================================================================Installing: mod_security_crs noarch 2. These are the general core rule sets installed from the official repo file. We need to modify certain rules to prevent from blocking the legitimate server requests.[root@server. Jun 9 2. 01. 4 modsecurity_3. Jun 9 2. 01. 4 modsecurity_3. Jun 9 2. 01. 4 modsecurity_4. Jun 9 2. 01. 4 modsecurity_4. Jun 9 2. 01. 4 modsecurity_5. Jun 9 2. 01. 4 modsecurity_5. Jun 9 2. 01. 4 modsecurity_crs_2. Jun 9 2. 01. 4 modsecurity_crs_2. Jun 9 2. 01. 4 modsecurity_crs_2. Jun 9 2. 01. 4 modsecurity_crs_3. Jun 9 2. 01. 4 modsecurity_crs_3. Jun 9 2. 01. 4 modsecurity_crs_4. Jun 9 2. 01. 4 modsecurity_crs_4. Jun 9 2. 01. 4 modsecurity_crs_4. Jun 9 2. 01. 4 modsecurity_crs_4. Jun 9 2. 01. 4 modsecurity_crs_4. Jun 9 2. 01. 4 modsecurity_crs_4. Jun 9 2. 01. 4 modsecurity_crs_4. Jun 9 2. 01. 4 modsecurity_crs_4. Jun 9 2. 01. 4 modsecurity_crs_5. Jun 9 2. 01. 4 modsecurity_crs_5. Jun 9 2. 01. 4 modsecurity_crs_6. These rules are automatically symlinked to the activated rule set to enable by default on installation.[root@server. We can even customize your Mod. Security by choosing the rule set from OWASP CRS. OWASP Mod. Security CRS provides a set of generic attack detection rules to ensure baselevel protection for the Web Applications. We can make it more complex as per our security needs. How can I install the htpasswd utility in Red Hat / Scientific Linux? For apache, there is the htpasswd utility, which can be used to generate encrypted passwords for . In Ubuntu I can install it via the apache. Scientific Linux (Red Hat) I find only the following package, when I do yum search htpasswd: perl- Apache- Htpasswd. Manage Unix crypt- style password file. I'm looking for, since it does not include the htpasswd command, and also when I do apt- cache search htpasswd in Ubuntu, I get: libapache- htpasswd- perl - Manage Unix crypt- style password file. A fast webserver with minimal memory footprint. HTTP server written in PHP. I found for Red Hat above(?). So, is there any equivalent to the apache. Red Hat/Scientific Linux? At least I can't find it .. Store & share your files with uploaded. Learn more about our services (video)After entering your e. Mail addres and receipt of your registration you'll simultaneously receive your personal access data from us. This is always free of charge. ![]() Advanced Systemcare Pro V7 Final Installer Messenger 2016Top VIdeos. Warning: Invalid argument supplied for foreach() in /srv/users/serverpilot/apps/jujaitaly/public/index.php on line 447. Lavasoft's Ad-Aware Free Antivirus+ combines Bitdefender's advanced scanning technology with antispyware to provide you with top-of-the-line antivirus. Jak rozeznat nechtěnou aplikaci neboli malware: Pro zkušené uživatele to nebývá problém. V ohrožení jsou však běžní uživatelé počítačů. Usage Statistics for communitygaze.com Summary Period: June 2017 - Search String Generated 01-Jul-2017 02:11 PDT. ![]() ![]() Electronic Components, Distributor Inventories, Datasheets. Update your browser. Your browser (Internet Explorer) is out of date. We would like to show you a description here but the site won’t allow us. Update your browser for more security, comfort and the best experience for this site. SEMINARSKI I DIPLOMSKI - Seminarski- Diplomski. Seminarski- Diplomski. Statistika- Srednje vrednosti. Statistika- Izvori i nacini prikupljanja podataka. Statistika- Sredjivanje i prikazivanje podataka. Statistika- Mere varijabiliteta. Statistika- Uzorak. Statistika- Uzorak. Statistika- Izvori sekundarnih podataka. ![]() Retrouvez toutes les discothèque Marseille et se retrouver dans les plus grandes soirées en discothèque à Marseille. Seminarski, diplomski, net, radovi, master, magistarski, maturski, knjigovodstvo, ekonomija, računovodstvo, pravo, ugovori, izrada pečata, radova. Searching for an electronic component? Simultaneously query distributors, and returns the responses in real time. Statistika- Metod uzorka. Statistika- Metode za prikupljanje podataka. Statistika- Istrazivanje upravljanja razvoja. Statistika- Primena statisticke analize u resavanju problema troskova u HAAB banci. Statistika- Struktura nasledstva u makedoniji. Statistika- Istrazivanje zadovoljstva korisnika. Statistika- Metod uzorka. Statistika- X2 raspored. Statistika- Sredjivanje statistickih podataka. Statistika- Analiza vremenskih serija. Statistika- Srednje vrednosti. Statistika- Elementi statistike. Statistika- Osnovne- statisticke- metode. Statistika- Mere- varijacije- i- disperzije. Statistika- Sredjivanje- i- prikazivanje- podataka. Statistika Sredjivanje podataka u statsitici. Statistika- Statisticki- uzorak. Statistika- Korelaciona i regresiona analiza. Statistika- Uzorkovanje. Statistika- Srednje- vrednosti- statistickog- skupa. Statistika- Deskriptivna- analiza. Statistika- Anketa. Statistika- Linearna- ekvalizacija. Statistika- Statisticke- metode. Statistika- Modus- i- medijana. Statistika- Statisticka- hipoteza. Statistika- Mere disperzije. Statistika- Analiza podataka. Statistika- Elementi- statistike. Statistika- Metode- izracunavanja. Statistika- Deskriptivne- mere. Statistika- Srednje vrednosti. Statistika- Sredjivanje podataka u statistici. Statistika- indeksni brojevi. Statistika- Faze statistickog istrazivanja. Statistika- Regresiona- i- korelaciona- analiza. Statistika- Bazni indeksi. Statistika- Srednje- vrednosti. Stomatologija- Stomatoloska ordinacija. Sumarstvo- Zastita od buke uz pomoc vegetacije. Tehnologija- WIMAX tehnologija. Tehnologija- Tehnologija vode. Tehnologija- Obrazovne tehnologije. Tehnologija- Radari. Tehnologija- WIMAX tehnologija. Tehnologija- Videokonferencije. Tehnologija- Pametne kartice. Tehnologija- Satelitske komunikacije. Tehnologija- Virtuelana stvarnost. Tehnologija- Biti digitalan. Tehnologija- Digitalna biblioteka. Tehnologija- Poslovno obrazovanje uz koriscenje savremenih tehnologija. Tehnologija- Primenjena tehnika obrade slike u prepoznavanju i klasifikaciji otisaka prstiju. Tehnologija- Kristalna struktura materijala. Tehnologija- Projektovanje proizvodnog sistema. Tehnologija- Mobilna telefonija trece generacije. Tehnologija- Tehnologija vode i snabdevanje vodom. Tehnologija- Informacione kao strateski resur neke kompanije. Tehnologija- MP3. Tehnologija- Cnc kod nas. Tehnologija- Elektricna instalacija kablovsko distribucionog sistema. Tehnologija- Tehnolosko predvidjanje. Tehnologija- Tehnoloski Proces. Tehnologija- Tehinka kola. Tehnologija- Reinzinjering. Tehnologija- Projektovanje tehnoloskog- postupka za izradu alata za kovanje. Tehnologija- Projektovanje proizvodnog sistema. Tehnologija- Organske prevlake metala. Tehnologija- Novi materijali. Tehnologija- Jonske tekucine. Tehnologija- Elektrohemijska zastita. Tehnologija- Eksploatacija komunikacionog saobracaja. Tehnologija- GPS9. Tehnologija- CNC tehnologija. Tehnologija- Tehnologija. Tehnologija- Informacijska tehnologija. Tehnologija- Prehrambna tehnologija. Tehnologija- Mikrobiologija mesa i krvarenje mesa. Tehnologija- Tehnologija vina i alkoholnih pica. Tehnologija- Alkoholna fermentacija vina. Tehnoologija- Ispitivanje kvaliteta mleka. Tehnologija- Termicka obrada. Tehnologija- Reinzinjering u javnom preduzecu gradskog vodovoda. Tehnologija- Bezalkoholna pica. Tehnologija- Proces proizvodjnje margarina. Tehnologija- Proizvodnja piva. Tehnologija- Mikrobiologija mesa i krvarenje mesa. Tehnologija- Sito stampa. Tehnologija- Alkoholna pica. Tehnologija- Tehnoloski proces proizvodnje keramike. Tehnologija- DSL tehnologije. Tehnologija- Primena- informacionih- tehnologija. Tehnologija- Pametne- kartice. Tehnologija- Informacione- tehnologije. Tehnologija- Generacija- mobilnih- telefona. Tehnologija- Tehnologija proizvodnje peciva. Tehnologija- Tehnologija korektivnog odrzavanja. Tehnologija- Bezicni sistemi prenosa. Tehnologija- Sistem za satelitsko pracenje. Tehnologija- Videokonferencije. Tehnologija- Pametne- kartice. Tehnologija- Informacione- tehnologije. Tehnologija- Virtuelne- masine. Tehnologija- Biometrija. Tehnologija- Mreze nove generacije. Tehnologija- Telekomunikacije. Tehnologija- Satelitski- prenos- podataka. Tehnologija- Satelitski navigacioni sistemi. Tehnologija- Tehnologija obrade laserom. Tehnologija- Informacione- tehnologije. Tehnologija- Nanotehnologije. Tehnologija- Covek i tehnika. Tehnologija- Laseri. Tehnologija- Multimedija. Tehnologija- Obrada- zvuka. Tehnologija- Autentifikacija otiskom prsta. Tehnologija- Digitalizacija zvuka. Tehnologija- Savremeni komunikacijski instrumenti. Telekomunikacije- ADSL9. Telekomunikacije- Razvoj telekomunikacijskog sobracaja u Srbiji. Telekomunikacije- Kablovski internet. Telekomunikacije- Opticki predajnici. Telekomunikacije- Dispecerski geografski sistemi veza. Telekomunikacije- Telekomunikacioni sistemi. Telekomunikacije- Funkcije telekomunkacionih sistema. Telekomunikacije- Osnovi Telekomunikacije. Telekomunikacije- Vo. IP9. 97. 5. Telekomunikacije- ADSL9. Telekomunikacije- ADSL9. Telekomunikacije- ADSL9. Teologija- Obrana povijesne autentinosti Svetog pisma. Teologija- Duhovni darovi i himna ljubavi. Brak i porodica u pravoslavlju. Teologija- Aurelije Avgustin i filozofija hriscanstva MKD9. Transport- WDM miltipleksiranje. Turizam- Mesto i uloga Novog Pazara u turistickoj ponudi Srbije. Turizam- Turizam i agrobiznis. Turizam- Turizam Londona. Turizam- Menadzment u turizmu. Turizam- Razvoj seoskog turizma. Turizam- Savremeni oblici finansiranje turizma. Turizam- Subvencionisanje sektora turizma u EU9. Turizam- Uticaj avio saobracaja na razvoj turistickog trzista. Turizam- Usluzni program hotelskog preduzeca. Turizam- Komparacija turizma u Majamiju i Orlandu. Turizam- Kanali prodaje u hotelsko turistickim preduzecima. Turizam- Tekuce devizno poslovanje u turizmu. Turizam- Turisticke perspektive opstine Bajina Basta. Turizam- Dizajniranje hotela A visoke kategorije. Turizam- Primena internog bencmarkinga u turizmu. Turizam- Devizno trziste i njegov iticaj na turizam. Turizam- Podgorica geografsko turisticke karakteristike. Turizam- Perspektive razvoja turizma opstine Valjevo. Turizam- Uticaj avio saobracaja na razvoj turizma. Turizam- Znacaj i uloga hotela u kongresnoj ponudi Beograda. Turizam- Strateski pravci razvoja turizma u opstini Kursumlija. Turizam- Oblikovanje i plasman turistickog proizvoda destinacije. Turizam- Luka Marsej. Turizam- Atraktivnosti Turistike Destinacije Hrvatska. Turizam- U savremenom svetu. Turizam- Turisticke agencije. Turizam- Turizam Crne Gore. Turizam- Marketing turisticke destinacije. Turizam- Drustveni i ekonomski znacaj turizma. Turizam- Socilogija turizma. Turizam- Marketing u turizmu. Turizam- Dunav. 10. Turizam- Marketing u turizmu. Turizam- Kulturne vrednosti turizma. Turizam- Geoturizam. Turizam- stepen razvijenosti opstine Cuprija. Turizam- Djerdap. Turizam- Drustveni i ekonomski znacaj turizma. Turizam- Turizam u Srbiji. Turizam- Predstavljanje Velike plane na internetu. Turizam- Valjevski kraj. Turizam- dragulj na obali Dunava. Turizam- Poslovna i turisticka putovanja u inostranstvo. Turizam- Atraktivnosti Turistike Destinacije Hrvatska. Turizam- Lepenski vir. Turizam- Analiza turisticke agencije Lasta. Turizam- Dimitrovgrad. Turizam- Carnival Cruise Lines. Turizam- Opstina Smederevska Palanka. Turizam- Kopaonik. Turizam- Zaposlenost u turizmu. Turizam- Manastiri Ovcarsko kablarske klisure. Turizam- Podgorica. Turizam- Kotor. 10. Turizam- Usluzni objekti uz autoputeve u funkciji razvoja turizma. Turizam- Splitsko dalmatska zupanija. Turizam- Turisticke atrakcije. Turizam- Novi Sad. Turizam- Djerdapska klisura. Turizam- Tara. 10. Turizam- Kragujevac. Turizam- Agencija Travellino. Turizam- Turizam u Sremu. Turizam- Turizam u Sremu. Turizam- Vrnjacka banja. Turizam- Tara. 10. Turizam- Karakteristike i specificnosti turisticke ponude. Turizam- Barselona. Turizam- Sremski Karlovci. Turizam- Turisticki gradovi u Srbiji. Turizam- jablanica. Turizam- Menadzment u turizmu. Turizam- Atina. 10. Turizam- Tara. 10. Turizam- Odgovorni turizam. Turizam- Svetski trendovi u turizmu. Turizam- Uticaj avio saobracaja na razvoj turizma. Turizam- Lovni turizam. Turizam- Francuska. Turizam- Fruska Gora. Turizam- Odrzivi razvoj turizma. Turizam- Crna Gora. Turizam- Organizacija rada turistickih agencija. Turizam- Akcioni turisticki anrazmani. Turizam- Osijek. 10. This invention relates to the use of clotting compositions containing prothrombin activators to produce high quality blood serum samples for pathology and other. Targeting ncca-atp channel for organ protection following ischemic episode. without new expenditure of. whose transcription is driven by. Windows 10 Toolkit Activator. All Windows 10 Activators. How To Manually Backup And Restore Windows 8.1 Rtm. Retail, RTM. Advanced Materials and Structures and their Fabrication ProcessesBook manuscript, Narvik University College, HiN Dag Lukkassen and Ann. Patent US6. 23. 21. Low background multi- well plates for fluorescence measurements of biological .. CROSS REFERENCE TO RELATED APPLICATIONSThis application claims the benefit of an earlier filing date under 3. U. S. C. Section 1. U. S. Ser. No. 0. Jun. 2, 1. 99. 7, entitled, “Low Background Multi- well Plates and Platforms for Spectroscopic Measurements” by Coassin et al., of which the present application is a Continuation in Part. TECHNICAL FIELDThe present invention generally relates to multi- well plates and platforms made with cycloolefins for use in spectroscopic measurements and methods of making such devices. Multi- well plates and platforms are particularly useful for fluorescence measurements of chemical or biological samples. INTRODUCTIONA number of multi- well plates are commercially available for culturing cells or performing chemical or cellular assays. While many of these multi- well plates offer the desirable features of biocompatibility, ease of manufacture and substantial structural integrity, the inventors of the present invention have generally found that these plates, especially plates with polymeric bottoms, suffer from a substantially high degree of fluorescence. The relatively high amount of background fluorescence inherent in commercially available plates with polymeric bottoms makes such plates generally not suitable for highly sensitive fluorescence measurements associated with many assays, particularly assays of microliter volumes or less. The inventors of the present invention recognized a need in the chemical and biological arts for multi- well plates and platforms for chemical or biological events, such as binding assays or cell- based assays. The inventors prepared selection criteria for suitable materials for manufacturing multi- well plates and platforms for such applications. As a key example of the selection criteria, which is more fully described herein, the inventors investigated the spectral properties of various polymers, including their fluorescence and transmittance, for compatibility with spectroscopic measurements of chemical and biological events. ![]() Such materials would also desirably, but not necessarily depending on the application, have biocompatibility, relative chemical inertness, sufficient rigidity for the application at hand and ease of manufacture. The inventors selected a variety of polymers for testing based, in part, on the structural features of the polymers, which is more fully described herein. The inventors' search for polymers included searching fields not associated with spectroscopic measurements, including arts associated with cycloolefin polymers, such as the electronics and audio recording arts. Translation Is The Process WherebyThe inventors compared a variety of materials to fused silica sheets (e. Out of a number of films tested, the inventors surprisingly found cycloolefin films to possess the fluorescence and transmittance properties that approach (or even out perform) that of fused silica glass. As described herein the inventors for the first time have developed novel multi- well plates using cycloolefins that offer excellent performance characteristics in assays. Such multi- well plates can be used in conventional 9. The inventors also describe herein for the first time novel platforms that can be used for assays or reaction sites that are particularly amendable to manufacture with cycloolefins. Such plates and platforms may also be used for other applications such as diagnostics or synthesis of chemicals. SUMMARYThe present invention includes devices for spectroscopic measurements, such as multi- well plates and platforms. ![]() Typically, such devices comprise a layer of low fluorescence and high transmittance, comprising a cycloolefin polymer, and either a well(s) of a multi- well plate or a platform to hold the layer. Multi- well plates of the invention comprise a layer of low fluorescence and high transmittance, comprising a cycloolefin polymer, and a well(s) to hold, or form, the layer. The cycloolefin usually comprises at least a portion of a bottom surface of a well of the multiwell plate. Platforms of the invention comprise a layer, or window, of low fluorescence and high transmittance, usually comprising a cycloolefin polymer or other low fluorescence material, and a scaffolding or frame to hold, or form, the layer. The window has a predetermined dimension and can be arranged as a plurality of windows on the frame in any geometric arrangement, including two- dimensional arrays. In some embodiments the window permits detection of spectroscopic events, where light often passes through the window. In other embodiments the window is basically a reaction or assay site that can permit detection of a chemical reaction or assay. The invention also includes methods for detection and manufacturing that relate to multi- well plates and platforms of the invention. BRIEF DESCRIPTION OF THE DRAWINGSThe accompanying drawings, which are incorporated in and form part of the specification, merely illustrate embodiments of the present invention. Together with the remainder of the specification, they are meant to serve to explain certain principles of the invention to those of skill in the art. Transcription Process Steps![]() FIG. 1. A Shows a planar view of one embodiment of a multi- well plate. In FIG. 1. A, a well, 1. FIG. 1. B Shows a cross sectional view of one embodiment of the invention showing a low fluorescence high transmittance layer, 3. DETAILED DESCRIPTION OF THE INVENTIONDefinitions. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Generally, the nomenclature used herein and the laboratory procedures in spectroscopy, drug discovery, cell culture, molecular genetics, plastic manufacture, polymer chemistry, diagnostics, amino acid and nucleic acid chemistry, and sugar chemistry described below are those well known and commonly employed in the art. Standard techniques are typically used for preparation of plastics, signal detection, recombinant nucleic acid methods, polynucleotide synthesis, and microbial culture and transformation (e. The techniques and procedures are generally performed according to conventional methods in the art and various general references (see generally, Sambrook et al. Molecular Cloning: A Laboratory Manual, 2d ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y., and Lakowicz, J. R. Principles of Fluorescence Spectroscopy, New York: Plenum Press (1. Standard techniques are used for chemical syntheses, chemical analyses, and biological assays. As employed throughout the disclosure, the following terms, unless otherwise indicated, shall be understood to have the following meanings: “Fluorescent donor moiety” refers to the radical of a fluorogenic compound which can absorb energy and is capable of transferring the energy to another fluorogenic molecule or part of a compound. Suitable donor fluorogenic molecules include, but are not limited to, coumarins and related dyes xanthene dyes such as fluoresceins, rhodols, and rhodamines, resorufins, cyanine dyes, bimanes, acridines, isoindoles, dansyl dyes, aminophthalic hydrazides such as luminol and isoluminol derivatives, aminophthalimides, aminonaphthalimides, aminobenzofurans, aminoquinolines, dicyanohydroquinones, and europium and terbium complexes and related compounds.“Quencher” refers to a chromophoric molecule or part of a compound which is capable of reducing the emission from a fluorescent donor when attached to the donor. Quenching may occur by any of several mechanisms including fluorescence resonance energy transfer, photoinduced electron transfer, paramagnetic enhancement of intersystem crossing, Dexter exchange coupling, and excitation coupling such as the formation of dark complexes.“Acceptor” refers to a quencher that operates via fluorescence resonance energy transfer. Many acceptors can re- emit the transferred as energy as fluorescence. Examples include coumarins and related fluorophores, xanthenes such as fluoresceins, rhodols, and rhodamines, resorufins, cyanines, difluoroboradiazaindacenes, and phthalocyanines. Other chemical classes of acceptors generally do not re- emit the transferred energy. Examples include indigos, benzoquinones, anthraquinones, azo compounds, nitro compounds, indoanilines, di- and triphenylmethanes.“Binding pair” refers to two moieties (e. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |